Transient Receptor Potential Melastatin 2 Enhances Vascular Reactivity During Development of Atherosclerosis in Mice

Abstract Background To investigate the function of transient receptor potential melastatin 2 (TRPM2) in vascular reactivity induced by 5-hydroxytryptamine (5-HT) aorta during development atherosclerosis mice. Methods Forty mice were randomly divided into 4 groups: C57BL/6J on normal diet (C57 + ND), high-fat HFD), apolipoprotein E gene knockout (ApoE−/−) ND (ApoE−/− and ApoE−/− HFD HFD). They fed with a or for 16 weeks. Aortic TRPM2 expression isometric contractions analyzed. Results In group, body weight, blood glucose, lipid concentrations increased, aortic plaques developed. Compared other 3 groups, mRNA protein levels significantly increased group (P < 0.01). to 5-HT was enhanced lower EC50 values. The inhibited inhibitors, N-p-amylcinnamoyl anthranilic acid (1 µmol/l) 2-aminoethyl diphenylborinate (10 µmol/l). Conclusions is upregulated ApoE HFD. Upregulation enhances atherosclerosis.